Next-generation phylogenetics takes root.

نویسندگان

  • John E McCormack
  • Brant C Faircloth
چکیده

It has been a tumultuous 5 years in phylogeography and phylogenetics during which both fields have struggled to harness the power of next-generation sequencing (NGS) (Ekblom & Galindo 2010; McCormack et al. 2012a). Fortunately, several methodological approaches appear to be taking root. In this issue of Molecular Ecology, O’Neill et al. (2013) employ one such method – parallel tagged sequencing (PTS) – to elucidate the phylogeography of a tiger salamander (Ambystoma tigrinum) species complex. This study demonstrates a practical application of NGS on a scale appropriate (and not overkill) for most biologists interested in phylogeography (~100 loci for ~100 individuals), and their results highlight several analytical challenges that lie ahead for researchers employing NGS techniques.

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عنوان ژورنال:
  • Molecular ecology

دوره 22 1  شماره 

صفحات  -

تاریخ انتشار 2013